Microinjection (Methods and Tools in Biosciences and Medicine)



Publisher: Birkhäuser Basel

Written in English
Cover of: Microinjection (Methods and Tools in Biosciences and Medicine) |
Published: Pages: 248 Downloads: 39
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Subjects:

  • Cellular biology,
  • Developmental biology,
  • Neurosciences,
  • Science/Mathematics,
  • Life Sciences - Biology - General,
  • methods,
  • Cytology,
  • Genetic Engineering,
  • Science,
  • Oncology,
  • Life Sciences - Cytology,
  • Science / Biology,
  • Biotechnology,
  • Technique,
  • Cytological Techniques,
  • Microinjections

Edition Notes

ContributionsJuan Carlos Lacal (Editor), Rosario Perona (Editor), James Feramisco (Editor)
The Physical Object
FormatHardcover
Number of Pages248
ID Numbers
Open LibraryOL9090446M
ISBN 103764359730
ISBN 109783764359737

Intuitive and ergonomic microinjections in Xenopus with the MK1 Micromanipulator from Singer. Esther Pearl and Marko Horb National Xenopus Resource, Marine Biological Laboratory, USA. Microinjection is an important technique used by many Xenopus researchers to inject into embryos for loss-of-function 1 and gain-of-function studies 2,3, making transgenic 4,5,6 and knock out lines 7, as well as. drummond microinjection apparatus parts price ust manual injector x nanoject + variable x + xv unit catalog # price size unit catalog # price size replacement glass ' replacement glass 8" ireplacement glass 7" •0" ring kit replacement plunger j6g/3y2" needle special "chip" =00tsw1tch g $ vial/ The zebrafish oocyte differs substantially from the zygote and cleavage-stage embryo with regard to the ease with which it can be microinjected with proteins or reagents that modify subsequent development. The objective of this chapter is to describe methods developed in this and other laboratories for microinjection and calcium imaging in the unfertilized zebrafish egg. Microinjection Molding of Enhanced Thermoplastics. By Mónica Oliveira, Victor Neto, Maria Fonseca, Tatiana Zhiltsova and José Grácio. Submitted: May 13th Reviewed: October 6th Published: March 28th DOI: /Cited by: 4.

Important information about our service - Please read. All you need to know: Go here for detailed information about our microinjection service (including sample shipment and payment). Sample preparation: Sample quality is the major factor that determines the success or failure of microinjection work. Please read our guidelines for sample preparation before sending your samples for microinjection. Dimethyl sulfoxide (DMSO) is commonly used as a solvent for water-insoluble drugs. Given that DMSO has varying cellular and behavioral effects ranging from increased membrane permeability to toxicity, microinjection of DMSO as a vehicle could confound the effects of other drugs. For example, DMSO is often used as a vehicle for studies examining the neurochemical mechanisms underlying morphine Cited by:   About this book Still the most comprehensive reference source on the development, production and therapeutic application of antibodies, this second edition is thoroughly updated and now has 30% more content. Microinjection is one of the leading methods for the production of transgenic fish. In this Application Note, we describe the sample preparation and the injection procedure which is performed with the aid of an Eppendorf Micromanipulator InjectMan NI 2 under a standard dissecting microscope. Fig. 1: Microinjection into early fish embryo.

VAT charges for Microinjection work We have been informed by the University of Cambridge Finance Division Tax Office that our Microinjection Service does not qualify for medical VAT-exmption. Consequently all microinjection charges for UK research groups (outside of the University of Cambridge) are subject to VAT at the standard rate (20%). / Electrical-assisted microinjection for analysis of fertilization and cell division in mammalian oocytes and early embryos. Mitosis and Meiosis Part A. editor / Helder Maiato ; Melina Schuh. Academic Press, pp. (Methods in Cell Biology).Cited by: 2. Transfer of embryos in a droplet of medium covered with silicon oil in the injection chamber; Fixation of an embryo at the holding pipette by negative pressure.

Microinjection (Methods and Tools in Biosciences and Medicine) Download PDF EPUB FB2

This detailed book explores how microinjection will be used in the foreseeable future, not only for generating animal models for biomedical research but also for changing economically or ecologically important species that can broadly impact our society in : Hardcover.

This detailed book explores how microinjection will be used in the foreseeable future, not only for generating animal models for biomedical research but also for changing economically or ecologically important species that can broadly impact our society in general.

The opening half of the book focuses on methods for generating mouse models, as. In Microinjection: Methods and Applications, expert researchers contribute methods utilizing microinjection techniques ranging from expression of RNA to the integration of DNA into the genome with the ultimate goal of learning about gene expression, signal.

DNA transformation and microinjection are essential tools for C. elegans research. Transformation is used to clone genes by mutant rescue, to over-express or ectopically express genes, to express tagged proteins, to study structure/function of protein domains, and to analyze DNA or RNA regulatory elements.

Furthermore, transgenes. Microinjection has shown to deliver adenoviral vectors effectively in a controlled manner to tumors (xenotransplants) in nude mice and also provides reproducible transfection results [].Microinjection has been very useful for producing recombinant cell lines; for example, green fluorescent protein (GFP) as a reporter gene effectively transferred to different cell lines like CHO DG44 and BHK.

Book Condition: This is an ex-library book and may have the usual library/used-book markings book has soft covers. With usual stamps and markings, In good all round condition.

Please note the Image in this listing is a stock photo and may not match the covers of the actual itemAuthor: Juan Carlos Lacal. In this book expert researchers contribute methods utilizing microinjection techniques ranging from expression of RNA to the integration of DNA into the genome with the ultimate goal of.

DNA microinjection methods are used to transfer genes between animals and are a popular technique for creating transgenic organisms, particularly mammals. DNA, or deoxyribonucleic acid, is the hereditary material in humans and almost all other organisms.

Nearly every. The book provides excellent informative updates on established techniques such as cRNA expression and examines new, cutting-edge technologies including antisense morpholino oligonucleotides, RNAi for knockdown experiments and the use of integrase to produce transgenic animals, all through microinjection : $ Microinjection remains the most popular and effective of the methods to introduce DNA, RNA, and proteins into fertilized zebrafish eggs.

The method is simple and reliable. A microinjection pipet is filled with the DNA or RNA solution and attached to an apparatus that Cited by: "The technique of needle microinjection has become a prominent experimental approach in biological research."--BOOK JACKET.

"This book presents information on the technique's principles, the required equipment, preparation of recipient cells and injected material and the analysis of the results. Microinjection Techniques: Injecting through the chorion I.

Introduction To investigate the role of a gene during development, overexpression or misexpression of your gene of interest is a fast assay. You can inject mRNA or DNA of the wild-type gene or a constitutively active form of the gene to investigate its gain-of-function phenotype.

Microinjection in zebrafish has been widely used for generating transgenic animals, and mapping cell fate in early blastula embryos 5,6,7,8,9. In addition, the application of this technique serves as a key step in generating germ-line chimeras by cell transplantation methods Cited by: C.

elegans microinjection. Contributed by Ian Chin-Sang, Queens University, ON, Canada. One way to make transgenic animals in C. elegans we use a microinjection technique. Briefly, a DNA construct (plasmid, cosmid or YAC) or PCR product with your gene(s) of interest is mixed with a co-injection marker and injected into the distal gonad (syncytium).

Serving scientist for over 50 years, WPI offers a variety of instruments for Zebrafish microinjection including pumps, pipetters, microscopes and more. One of our most popular pumps for microinjection is the PV Pneumatic PicoPump. The PV and PV, Pneumatic PicoPumps, were designed to simplify intracellular injection.

You get repeatable. Since there is a new microinjection laboratory working onthis illing and microinjection were developed jointly, improving theirsynergies and. MICROINJECTION • Microinjection where the DNA is directly injected into plant protoplasts or cells (specifically into the nucleus or cytoplasm) using fine tipped ( - micrometer diameter) glass needle or micropipette.

High–quality microinjection results require precision and reliability. Eppendorf's electronic microinjectors, the FemtoJet ® 4i and FemtoJet 4x, are your tools of choice for reproducibly injecting small volumes into adherent cells and suspension cells.

In combination with the TransferMan ® 4r or InjectMan ® 4 they form an ideal system to facilitate and speed up your workflow. THE ZEBRAFISH BOOK A guide for the laboratory use of zebrafish Danio (Brachydanio) rerio by Monte Westerfield, Institute of Neuroscience, University of Oregon.

In this book expert researchers contribute methods utilizing microinjection techniques ranging from expression of RNA to the integration of DNA into the genome with the ultimate goal of learning about gene expression, signal transduction, and protein function.

Methods in Molecular Biology: Microinjection: Methods and Applications (Hardcover). Product Information. The technique of needle microinjection has become a prominent experime ntal approach in biological research.

Cellular organelles, DNA and RNA, enzymes, structural proteins, metabolites, ions and antibodies are j ust some of the molecular and cellular elements that have been transpo sed from test tubes into living cells by needle injection.

T1 - Microinjection of Xenopus embryos. AU - Garriock, R. AU - Krieg, P. PY - /1/1. Y1 - /1/1. N2 - INTRODUCTION Microinjection of Xenopus embryos is an important technique with multiple applications in the fields of Cell Biology and Developmental : R.

Garriock, Paul A Krieg. Book Appointment BOTOX ® relaxes muscles, and thereby erasing wrinkles for a youthful appearance. This injectable treatment derived from a purified protein smoothes out lines by preventing the muscles from contracting into a wrinkle. Microinjection is a powerful technique for gene manipulation, and thus it undoubtedly is one of useful approaches in the studies of gene function and embryonic development.

This detailed book explores how microinjection will be used in the foreseeable future, not only for generating animal models for biomedical research but also for changing economically or ecologically important species that can broadly impact our society in general.

Book Overview. Altmetric Badge. Chapter 1 Microinjection and Micromanipulation: A Historical Perspective Chapter 16 Microinjection and Oviduct Transfer Procedures for Rat Model Generation with CRISPR-Cas9 Technology Altmetric Badge.

Chapter 17 Molecular Aspects of Zinc Finger Nucleases. Microinjection is a powerful tool for studying embryonic development and analyzing gene functions in fish.

This technique was first applied to model species of fish such as zebrafish and medaka. Microinjection-Based RNA Interference Method in the Water Flea, Daphnia pulex and Daphnia magna. By Kenji Toyota, Shinichi Miyagawa, Yukiko Ogino and Taisen Iguchi. Submitted: May 14th Reviewed: September 10th Published: April 6th DOI: /Cited by: 3.

Protocol Microinjection of RNA into Mouse Zygotes / This is a free sample of content from Manipulating the Mouse Embryo: A Laboratory Manual, Fourth edition Click here for more information or to buy the book. © by Cold Spring Harbor Laboratory Press. Microinjection is an effective method for creating transgenic animals, for RNAi of selected genes, and for introducing various types of molecules directly to cells.

For DNA transformation, the easiest approach is to inject DNAs into the distal arm of the gonad (; Mello et al., ; Mello and Fire, ). We have developed a new inexpensive and easy method to position zebrafish embryos for microinjection. Our technique is a simplification of the method published by Eric S.

Weinberg (Zebrafish Science Monitor 2 (1)) and of the method taught by Manfred Schartl in the course "Gene Expression and Regulation in Laboratory Fish", Würzburg.Cell microinjection is a technique of precise delivery of substances into cells and is widely used for studying cell transfection, signaling pathways, and organelle functions.

Microinjection of the embryos of zebrafish, the third most important animal model, has become a very useful technique in bioscience. However, factors such as the small cell size, high cell deformation tendency, and Cited by: 9. Microinjection into the yolk cell of zebrafish eggs is feasible, but the application of exact volumes appears problematic.

However, microinjection is a powerful tool for studies without the demand for high volume accuracy. Adopting microinjection for pollutant research requires further by: 9.